Electrospray ionization mass spectrometric analysis of subunits of NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria.
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چکیده
8 Karas, M., Rahr, U., Ingendoh, A. I., Nordhoff, E., Stahl, R., Strupat, K. and Hillenkamp, F. (1990) Anal. Chim. Acta 241,175-185 9 Hefta. S. A,, Stahl, D. C., Mahrenholz, A. M., Martino, 1’. A,, Kutherford, S. M. and Lee, T. D. (1991) I’roc. Annu. Meet. Am. SOC. Mass Spectrom. 141 614 17 10 Chevrier, M. R. and Cotter, K. J. (1991) Proc. Annu. Meet. Am. Soc. Mass Spectrom. 933-934 11 Mock, K. K., Sutton, C. W. and Cottrell, J. S. (1992) Rapid Commun. Mass Spectrom. 6.233-238 12 Zaluzec. E. J., Gage, D. A., Allison, J. and Watson, J. T. (1993) Proc. Annu. Meet. Am. SOC. Mass Spectrom. 436a-436b 13 Corran, P. H. and Waley, S. G. (1975) Hiochem. J.
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 22 2 شماره
صفحات -
تاریخ انتشار 1994